Streptomyces montanus sp. nov., a novel actinomycete isolated from soil

A novel actinomycete, designated strain NEAU-C151 T , was isolated from soil collected from Mount Song and characterized using a polyphasic approach. Analysis of the 16S rRNA gene sequence indicated that strain NEAU-C151 T belongs to the genus Streptomyces and exhibited 97.5, 97.4 and 97.4 % similarities to Streptomyces lincolnensis NRRL 2936 T , Streptomyces coacervatus AS-0823 T , and Streptomyces longisporus ISP 5166 T , respectively. The assignment of strain NEAU-C151 T to the genus Streptomyces was confirmed by chemotaxonomic data: anteiso -C 15 : 0 , C 16 : 0 , iso -C 16 : 0 , C 16 : 1 (ω7c) and anteiso -C 17 : 0 as the major cellular fatty acids; whole-cell sugars contained ribose and glucose; phospholipid profile consisted of diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), unidentified phospholipid (PL), unidentified lipids (L) and phosphatidylinositol mannoside (PIM); the menaquinones were MK-9(H 4 ), MK-9(H 6 ), MK-10(H 2 ) and MK-9(H 8 ). However, multilocus sequence analysis based on five other house-keeping genes ( atp D , gyr B , rec A, rpo B , and trp B), DNA–DNA relatedness and phenotypic data showed that strain NEAU-C151 T could be distinguished from its closest relatives. Consequently, strain NEAU-C151 T represents a novel species of the genus Streptomyces , for which the name Streptomyces montanus sp. nov. is proposed. The type strain is NEAU-C151 T (=CGMCC 4.7498 T =DSM 107808 T ).