Open AccessLuteimonas chenhongjianii, a novel species isolated from rectal contents of Tibetan Plateau pika (Ochotona curzoniae)
Author(s) -
Zhang Gui,
XinHe Lai,
Jing Yang,
Dong Jin,
Ji Pu,
Yanwen Xiong,
Caixin Yang,
Kui Dong,
Ying Huang,
Xu Luo,
Shan Lu,
Jianguo Xu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004151
Subject(s) - biology , pika , 16s ribosomal rna , phylogenetic tree , microbiology and biotechnology , strain (injury) , bacteria , gene , anatomy , ecology , biochemistry , genetics , national park
Two Gram-stain-negative, strictly aerobic, bright-yellow-pigmented and rod-shaped bacteria (strains 100069 and 100111 T ) with a single polar flagellum were isolated from the rectal contents of plateau pika ( Ochotona curzoniae ). Based on the results of nearly full-length 16S rRNA gene sequence and phylogenetic analyses, strains 100069 and 100111 T belong to the genus Luteimonas , and are closest to Luteimonas rhizosphaerae 4-12 T (98.02 % similarity), Luteimonas aestuarii B9 T (97.8 %) and Luteimonas terrae THG-MD21 T (97.74 %). The DNA G+C contents of these two isolates were 68.30 mol% and 68.29 mol%, respectively. The highest average nucleotide identity (ANI) value between strain 100111 T and its closely related species was 83.34 %, well below the threshold of 95–96 %. The major cellular fatty acids were iso-C 11 : 0 , iso-C 15 : 0 and iso-C 17 : 1 ω9. Polar lipid content was dominated by diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid and an unidentified lipid. Ubiquinone-8 (Q-8) was the predominant respiratory quinone. These two isolates grew optimally at 35–37 °C, pH 7.0–8.0 and with 1.0 % (w/v) NaCl. The results of ANI analysis and other characteristics obtained from our polyphasic study showed that strains 100069 and 100111 T represent a novel species in genus Luteimonas , for which the name Luteimonas chenhongjianii sp. nov. (type strain 100111 T =DSM 104077 T =CGMCC 1.16429 T ) is proposed.