Open AccessSerinicoccus hydrothermalis sp. nov., isolated from shallow-sea hydrothermal systems off Kueishantao Island
Author(s) -
Qingfeng Zhang,
Jianing Ye,
Limei Yu,
Dan Lin,
Kai Tang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004145
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , phosphatidylglycerol , microbiology and biotechnology , diamino acid , ribosomal rna , phylogenetics , gene , biochemistry , phospholipid , phosphatidylcholine , gene sequence , anatomy , membrane
A Gram-stain-positive, non-flagellated, non-gliding, coccoid bacterial strain, designated JLT9 T , was isolated from the shallow-sea hydrothermal system off Kueishantao Island, Taiwan, ROC. Strain JLT9 T was aerobic, chemoheterotrophic and grew optimally at 35 °C, at pH 6.0 and in the presence of 2.5 % (w/v) NaCl. Strain JLT9 T exhibited highest 16S rRNA gene sequence similarity to Serinicoccus marinus DSM 15273 T (98.83 %). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain JLT9 T belonged to the genus Serinicoccus , clustering with Serinicoccus marinus JC1078 T , Serinicoccus profundi MCCC 1A05965 T , Serinicoccus sediminis GP-T3-3 T and Serinicoccus chungangensis CAU9536 T . The digital DNA–DNA genome hybridization values between strain JLT9 T and the closest related strain S. marinus DSM 15273 T was 34.30 %. The DNA G+C content was 72.43 mol%. The dominant fatty acids were identified as iso-C 15 : 0 (41.4 %) and iso-C 16 : 0 (24.7 %). The polar lipids of strain JLT9 T comprised diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, three unidentified glycolipid and an unidentified phospholipid. The predominant isoprenoid quinone was MK-8 (H 4 ). The cell wall contained ornithine and serine, and no diaminopimelic acid. On the basis of phylogenetic data and several distinct phenotypic characteristics, strain JLT9 T represents a novel species of the genus Serinicoccus , for which the name Serinicoccus hydrothermalis sp. nov. is proposed. The type strain is JLT9 T (=CGMCC 1.15779 T =JCM 31502 T ).