Open AccessMesohalobacter halotolerans gen. nov., sp. nov., isolated from a marine solar saltern
Author(s) -
Xi Feng,
Jinyu Zhang,
Jin Sang,
DaShuai Mu,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004143
Subject(s) - biology , microbiology and biotechnology , botany
A Gram-stain-negative, non-motile, fine rod or short filament shaped, jacinth pigmented bacterium, designated strain WDS2C27 T , was isolated from a marine solar saltern in Wendeng, Weihai, PR China (37°31′5″ N, 122°1′47″ E). Growth of WDS2C27 T occurred at 20–42 °C (optimum 37 °C) and pH 6.5–8.5 (optimal pH 7.0–8.0). Optimal growth occurred in modified marine broth containing 6 % (w/v) NaCl. The major polar lipids in WDS2C27 T were phosphatidylethanolamine, two unidentified aminolipids and one unidentified lipid. The major respiratory quinone of WDS2C27 T was MK-6. The dominant fatty acids were iso-C 15 : 0 and anteiso - C 15 : 0 . The DNA G+C content was 35.0 mol%. The nucleotide sequence of the 16S rRNA gene indicated that the most closely related strain was Psychroflexus planctonicus X15M-8 T (92.0 % over 1452 bp). WDS2C27 T showed 60.7 % average amino acid identity, 55.6 % percentage of conserved proteins, 75.0 % average nucleotide identity and 13.1 % digital DNA–DNA hybridization identity with the type species of the genus Psychroflexus , Psychroflexus torquis ATCC 700755 T . The phenotypic and genotypic properties and phylogenetic inference indicated that WDS2C27 T could be assigned to a novel species within a novel genus, for which the name Mesohalobacter halotolerans gen. nov., sp. nov. is proposed. Strain WDS2C27 T (=MCCC 1H00133 T =KCTC 52044 T ) is the type strain.