Streptomyces lasalocidi sp. nov. (formerly ‘Streptomyces lasaliensis’), an actinomycete isolated from soil which produces the polyether antibiotic lasalocid

Strain ATCC 31180 T was isolated from soil collected in Hyde Park, Massachusetts (USA), and found to produce the polyether antibiotic lasalocid. The name ‘ Streptomyces lasaliensis ’ has been in common use since 1974, without a recognized taxonomic description. The most closely related type cultures determined by rRNA gene sequence similarity were Streptomyces longwoodensis DSM 41677 T (100 %) and Streptomyces galbus DSM 40089 T (100 %). OrthoANI values with S. longwoodensis and S. galbus were 95.50 and 94.41 %, respectively. Chemotaxonomic characteristics supported inclusion within the genus Streptomyces . The cell wall peptidoglycan contained ll -diaminopimelic acid, and the major whole-cell sugars were glucose and ribose. Polar lipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylglycerol, one unidentified lipid and one unidentified glycolipid. The major menaquinones detected were MK9(H 4 ), MK9(H 6 ) and MK9(H 8 ). The major cellular fatty acids were anteiso - C 15 : 0 , anteiso - C 17 : 0 , iso - C 16 : 0 , iso - C 15 : 0 and anteiso - C 17 : 1 . Its DNA had a G+C content of 72.6 %. Differentiation of ATCC 31180 T from the closely related species was evident from digital DNA–DNA hybridization values of 61.80 and 56.90 % for S. longwoodensis and S. galbus respectively. Significant differences were seen in the polyphasic phenotypic analyses. ATCC 31180 T produced lasalocid, grew from 10 to 45 °C, pH4-8 and in the presence of 0–10 % NaCl, 0.01 % NaN 3 and 1 % phenol. Melanin was produced; H 2 S and indole were not. Nitrate was not reduced. Spore chains were retinaculum-apertum and spore surfaces were smooth. Spore colour, mycelia colour and soluble pigment production were medium-dependent. The proposed name is Streptomyces lasalocidi sp. nov.; the type strain being ATCC 31180 T (=NRRL 3382 T =DSM 46487 T ).