Open AccessParaglaciecola marina sp. nov., isolated from marine alga (Sargassum natans (L.) Gaillon)
Author(s) -
Yan Wang,
Yao Zhang,
Tingwei Liu,
Xiaoli Zhu,
Jin Ma,
Xin Su,
Jing Kang,
Changjiang Guo
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004132
Subject(s) - biology , 16s ribosomal rna , strain (injury) , botany , phylogenetic tree , microbiology and biotechnology , bacteria , gene , anatomy , biochemistry , genetics
A novel Gram-stain-negative, straight or curved rod-shaped, non-spore-forming, strictly aerobic, motile bacterium with a single polar flagellum, designated D3211 T , was isolated from marine alga collected at the seashore of Yantai, PR China. The organism grew optimally at 24 °C, pH 7.0 and in the presence of 2.0 % (w/v) NaCl. Strain D3211 T contained ubiquinone 8 as the major respiratory quinone and C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c , C 16 : 0 , iso-C 17 : 0 and anteiso-C 17 : 1 B and/or iso-C 17 : 1 I as the major fatty acids. The predominant polar lipids of strain D3211 T were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The DNA G+C content of strain D3211 T was 39.4 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that the novel strain was related most closely to Paraglaciecola arctica BSs20135 T , Paraglaciecola aestuariivivens JDTF-33 T , Paraglaciecola aquimarina KCTC 32108 T , Paraglaciecola mesophila DSM 15026 T , Paraglaciecola psychrophila JCM 13954 T and Paraglaciecola polaris ARK 150 T with 97.6, 97.6, 97.5, 97.4, 97.3 and 97.1 % sequence similarities, respectively. Calculated average nucleotide identity and DNA–DNAhybridization values between strain D3211 T and its phylogenetically related Paraglaciecola species were in the range 70.2–73.4 % and 19.1–20.4 %, respectively. On the basis of polyphasic analyses, strain D3211 T represents a novel species of the genus Paraglaciecola , for which the name Paraglaciecola marina sp. nov. is proposed. The type strain is D3211 T (=KCTC 72122 T =MCCC 1K03603 T ).