Marinobacter changyiensis, sp. nov., isolated from offshore sediment

An aerobic, Gram-stain-negative bacterium, designated CLL7-20 T , was isolated from a marine sediment sample from offshore of Changyi, Shandong Province, China. Cells of strain CLL7-20 T were rod-shaped, motile with one or more polar flagella, and grew optimally at pH 7.0, at 28 °C and with 3 % (w/v) NaCl. The principal fatty acids of strain CLL7-20 T were C 16 : 0 and summed feature 3 (C 16 : 1 ω 7 c /C 16 : 1 ω 6 c ). The main polar lipids of strain CLL7-20 T were phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG) and an unidentified aminolipid (AL). Strain CLL7-20 T contained Q-9 as the major respiratory quinone. The G+C content of its genomic DNA was 56.2 mol%. Phylogenetically, strain CLL7-20 T branched within the genus Marinobacter , with M. daqiaonensis YCSA40 T being its closest phylogenetic relative (96.7 % 16S rRNA gene sequence similarity), followed by M. sediminum R65 T (96.6 %). Average nucleotide identity and in silico DNA–DNA hybridization values between strain CLL7-20 T and the closest related reference strains were 73.2% and 19.8 %, respectively. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, we suggest that strain CLL7-20 T (=MCCC 1A14855 T =KCTC 72664 T ) is the type strain of a novel species in the genus Marinobacter , for which the name Marinobacter changyiensis sp. nov. is proposed. Based on the genomic analysis, siderophore genes were found from strain CLL7-20 T , which indicate its potential as a promising alternative to chemical fertilizers in iron-limitated environments such as saline soils.