Open AccessNovosphingobium silvae sp. nov., isolated from subtropical forest soil
Author(s) -
Guang-Da Feng,
Wendi Chen,
Jun Zhang,
Yonghong Wang,
Yang Liu,
Yang Zhou,
Honghui Zhu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004115
Subject(s) - biology , phylogenetic tree , 16s ribosomal rna , strain (injury) , tropical and subtropical moist broadleaf forests , spermidine , botany , microbiology and biotechnology , subtropics , gene , biochemistry , ecology , anatomy , enzyme
A novel bacterial strain, designated FGD1 T , was isolated from subtropical forest soil of the Nanling National Forest Park located in Guangdong Province, P.R. China. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain FGD1 T was most closely related to Novosphingobium lindaniclasticum DSM 25049 T (98.8 %), followed by N. barchaimii DSM 25411 T (98.7 %), N. guangzhouense DSM 32207 T (98.2 %), N. panipatense DSM 22890 T (98.1 %) and other species of Novosphingobium (<98 %). The draft genome sequence was 4.58 Mb in length with a G+C content of 65.1 mol%. The calculated average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain FGD1 T and closely related type strains were 77.7‒79.6 % and 21.7–22.9 %, respectively. Major fatty acids were summed feature 8 (C 18 : 1 ω 7c and/or C 18 : 1 ω 6c ), summed feature 3 (C 16 : 1 ω 7c and/or C 16 : 1 ω 6c ), C 14 : 0 2-OH and C 16 : 0 . The predominant respiratory quinone was ubiquinone 10 and the major polyamine was spermidine. Polar lipids were composed of sphingoglycolipid, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, an unidentified phospholipid and lipid. The polyphasic taxonomic results indicated that strain FGD1 T represents a novel species of the genus Novosphingobium , for which the name Novosphingobium silvae sp. nov. is proposed. The type strain is FGD1 T (=GDMCC 1.1761 T =KACC 21283 T ).