Open AccessStreptomyces tailanensis sp. nov., an actinomycete isolated from riverside silt
Author(s) -
Baozhi Sun,
Zhan-Feng Xia,
Linlin Yuan,
ChuanXing Wan
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004105
Subject(s) - biology , 16s ribosomal rna , strain (injury) , streptomyces , mycelium , muramic acid , diamino acid , microbiology and biotechnology , actinomycetales , phosphatidylinositol , botany , bacteria , peptidoglycan , gene , biochemistry , cell wall , genetics , gene sequence , kinase , anatomy
A novel actinobacterium, designated TRM68348 T , was isolated from the silt collected from the Tailan River in Xinjiang Province, north-west China. The strain was aerobic and Gram-stain-positive. The aerial mycelium was densely straight or tortuous, with a few branches of hyphae and no spores. The whole-cell sugar pattern of strain TRM68348 T consisted of ribose and glucose. The diagnostic diamino was ll -diaminopimelic acid. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylinositol mannose and an unidentified phospholipid. The predominant menaquinones were MK-9 (H 10 ), MK-9 (H 6 ) and MK-9 (H 2 ). The major fatty acids (>5 %) were iso-C 14 : 0 , iso-C 15 : 0 , anteiso-C 15 : 0 , iso-C 16 : 0 , C 16 : 0 and summed feature 6. The G+C content of the genomic DNA was 69.93 mol%. Phylogenetic analysis showed that strain TRM68348 T shared 16S rRNA gene sequence similarity of 98.14 % to the closest described species Streptomyces capitiformicae 1H-SSA4 T . Strain TRM68348 T had a relatively low DNA–DNA relatedness value with S. capitiformicae 1H-SSA4 T as determined by calculating the average nucleotide identity value (92.78 %). Strain TRM68348 T could also be differentiated from S. capitiformicae 1H-SSA4 T based on morphological and physiological characteristics. On the basis of the evidence from this polyphasic study, the strain is concluded to represent a novel species of the genus Streptomyces , for which the name Streptomyces tailanensis sp. nov. is proposed. The type strain is TRM68348 T (=CCTCC AA 2018086 T =KCTC 49274 T ).