Open AccessPseudomonas piscis sp. nov., isolated from the profound head ulcers of farmed Murray cod (Maccullochella peelii peelii)
Author(s) -
Yang Liu,
Qiuhua Rao,
Jochen Blom,
Lin Qiu,
Tuyan Luo
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004101
Subject(s) - biology , 16s ribosomal rna , pseudomonas , phylogenetic tree , phylogenetics , microbiology and biotechnology , strain (injury) , botany , bacteria , gene , genetics , anatomy
A Gram-stain-negative, strictly aerobic, motile, rod-shaped bacterium with monopolar flagella, designated as MC042 T , was isolated from the profound head ulcers of farmed Murray cod sampled from Zhejiang Province, China. Analysis of its 16S rRNA gene sequence and multilocus sequence analysis phylogeny showed that strain MC042 T belonged to the genus Pseudomonas , showing the highest 16S rRNA gene sequence similarity to Pseudomonas juntendi BML3 T (98.9 %), and less than 98.8 % similarity to other Pseudomonas species with validly published names. Whole-genome sequencing and phylogenetic reconstruction based on a core set of 1563 Pseudomonas genes further indicated that strain MC042 T was most closely related to the clade formed by Pseudomonas protegens CHA0 T and Pseudomonas saponiphila DSM 975 T and distantly related to any of the validly published species of the genus Pseudomonas . Furthermore, strain MC042 T could be distinguished from its closely related species of the genus Pseudomonas by its ability to assimilate maltose, d -xylose and melibiose, but not d -mannitol. The principal fatty acids were C 16 : 0 , summed feature 3 (iso-C 15 : 0 2-OH and/or C 16 : 1 ω 7c ) and summed feature 8 (C 18 : 1 ω 7 c and/or C 18 : 1 ω 6 c ). The respiratory quinone was Q-9. Polar lipids of strain MC042 T comprised diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, unidentified glycolipids, an unidentified lipid, an unknown glycolipid and aminolipid. Based on its phenotypic, chemotaxonomic and phylogenetic features, strain MC042 T is considered to represent a novel species, for which the name Pseudomonas piscis sp. nov. is proposed. The type strain is MC042 T (=KCTC 72033 T =MCCC 1K03575 T ).