Nocardioides euryhalodurans sp. nov., Nocardioides seonyuensis sp. nov. and Nocardioides eburneiflavus sp. nov., isolated from soil

Three aerobic, rod-shaped actinobacterial strains, designated MMS17-SY117 T , MMS17-SY207-3 T and MMS17-SY213 T , were isolated from soil and their taxonomic positions were analysed using a polyphasic approach. The isolates showed best growth at 30 °C, pH 7 and 0–1 % (w/v) NaCl. On the basis of 16S rRNA gene sequence similarity, the isolates were affiliated to the genus Nocardioides , and the closest species to MMS17-SY117 T , MMS17-SY207-3 T and MMS17-SY213 T were Nocardioides aestuarii JC2056 T (97.76%), Nocardioides currus IB-3 T (97.41%) and Nocardioides exalbidus RC825 T (98.71%), respectively. Each isolate formed a distinct cluster within the Nocardioides clade in the phylogenetic tree. The orthologous average nucleotide identity and digital DNA–DNA hybridization values were in the range of 74.4–85.7 % and 16.6–39.2 %, respectively, with the type strains of related species. The major polar lipids in all three strains were phosphatidylinositol, phosphatidylglycerol and diphosphatidylglycerol. The predominant fatty acids were iso-C 16 : 0 and C 17 : 1  ω8 c . MK-8(H 4 ) was the major isoprenoid quinone and ll -DAP was the major diamino acid. Galactose, glucose and rhamnose were present in the whole-cell hydrolysate, and MMS17-SY213 T also contained mannose and ribose. The DNA G+C contents of MMS17-SY117 T , MMS17-SY207-3 T and MMS17-SY213 T were 72.2, 70.4 and 71.5 mol%, respectively. The phylogenetic, phenotypic and chemotaxonomic data supported the classification of each strain as representing a new species of Nocardioides , for which the names Nocardioides euryhalodurans sp. nov. (MMS17-SY117 T =KCTC 49175 T =JCM 32831 T ), Nocardioides seonyuensis sp. nov. (MMS17-SY207-3 T =KCTC 49176 T =JCM 32832 T ) and Nocardioides eburneiflavus sp. nov. (MMS17-SY213 T =KCTC 49177 T =JCM 32833 T ) are proposed accordingly.