Open AccessVitreimonas flagellata gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from an activated sludge sample
Author(s) -
Mipeshwaree Devi Asem,
Nimaichand Salam,
Wei Zheng,
Lihua Liu,
Xiaotong Zhang,
Ming-Xian Han,
JianYu Jiao,
Feng Guo,
Wenjun Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004082
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , glycolipid , microbiology and biotechnology , phylogenetics , botany , gene , genetics , biochemistry , anatomy
A coccobacilli-shaped proteobacterium, designated strain SYSU XM001 T , was isolated from an activated sludge sample collected from JiMei sewage treatment plant, Xiamen, PR China. Cells were Gram-stain-negative, catalase-positive and oxidase-negative. The 16S rRNA gene sequence of strain SYSU XM001 T shared less than 92 % sequence identities with members of the class Alphaproteobacteria , with highest sequence similarity to Aquidulcibacter paucihalophilus TH1-2 T (91.6 %; family Hyphomonadaceae , order Rhodobacterales ). The strain exhibited growth at 25–37 °C, pH 7.0–9.0 and in the presence of up to 1 % (w/v) NaCl. Its chemotaxonomic features included ubiquinone-10 as the respiratory isoprenologue, iso-C 16 : 0 , 10-methyl C 16 : 0 TSBA and anteiso-C 17 : 0 as major cellular fatty acids and monoglycosyldiglyceride, glucuronopyranosyldiglyceride and two unidentified glycolipids as the main polar lipids. The DNA G+C content was determined to be 62.9 % (draft genome). Analyses of the phylogenetic data and differences in the chemotaxonomic and biochemical features from related genera in the family Hyphomonadaceae indicated that strain SYSU XM001 T merits representation of a novel species of a novel genus, for which the name Vitreimonas flagellata gen. nov., sp. nov. is proposed. The type strain of Vitreimonas flagellata is SYSU XM001 T (=CGMCC 1.16661 T =KCTC 62915 T ).