Open AccessGenomic and genetic sequence information of strains assigned to the genus Rhodopseudomonas reveal the great heterogeneity of the group and identify strain Rhodopseudomonas palustris DSM 123T as the authentic type strain of this species
Author(s) -
Johannes F. Imhoff,
Terrance E. Meyer,
John A. Kyndt
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004077
Subject(s) - rhodopseudomonas palustris , biology , rhodopseudomonas , strain (injury) , 16s ribosomal rna , microbiology and biotechnology , botany , bacteria , genetics , anatomy
The genus Rhodopseudomonas , containing purple nonsulfur photosynthetic Proteobacteria, has a number of strains that belong to different species, although many of them are collectively called Rhodopseudomonas palustris . The type species R. palustris and closely related species are the focus of this paper. The comparison of available genome sequences indicate that the following Rhodopseudomonas species are well recognized: R. palustris (strains ATH 2.1.6 T =DSM 123 T =NBRC 100419 T and BisB5), Rhodopseudomonas rutila (strains R1 T , DSM 126, CGA009, ATH 2.1.37, Eli 1980, ATCC 17001 and TIE1), Rhodopseudomonas pentothenatexigens JA575 T and Rhodopseudomonas faecalis JCM 11668 T . Other strains for which genome sequences are available are distinct from these four species. Evidence is presented that R. palustris strain ATH 2.1.6 T –KCM as obtained directly from the van Niel collection by one of us (T.E.M.) is identical to the DSMZ deposit DSM 123 T of ATH 2.1.6 T , but not to the deposit at ATCC 17001. The amino acid sequences of the cytochromes C2 and C556 from R. palustris strain ATH 2.1.6 T –KCM are in complete agreement with the translated genome sequences of R. palustris DSM 123 T . In addition, the 16S rRNA gene sequence of R. palustris NBRC 100419 T completely matches that of strain DSM 123 T . In conclusion, the type strain of R. palustris ATH 2.1.6 T is correctly represented by DSM 123 T and NBRC 100419 T . However, the deposit at ATCC 17001 has properties that do not conform with properties of authentic R. palustris , but rather indicate that this is a strain of R. rutila . The previously suggested assignment of the type strain of R. palustris DSM 123 T to the new species R. pseudopalustris was incorrect because strain DSM 123 T is the authentic type strain of R. palustris .