Open AccessWeissella sagaensis sp. nov., isolated from traditional Chinese yogurt
Author(s) -
Yu Qin Li,
Wen Tian,
Chun Tao Gu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004062
Subject(s) - weissella , biology , housekeeping gene , 16s ribosomal rna , microbiology and biotechnology , gene , sequence analysis , strain (injury) , in silico , bacteria , genetics , leuconostoc , lactic acid , gene expression , anatomy
Three Gram-stain-positive bacterial strains, designated X0750 T , X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 16S rRNA gene and three housekeeping genes ( pheS , rpoA and recA ), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), in silico DNA–DNA hybridization ( is DDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750 T was phylogenetically related to the type strains of Weissella hellenica , Weissella bombi , Weissella paramesenteroides , Weissella jogaejeotgali , Weissella thailandensis , Weissella oryzae , Weissella cibaria and Weissella confusa , having 94.4–100 % 16S rRNA gene sequence similarities, 76.7–90.0 % pheS gene sequence similarities, 88.9–99.4 % rpoA gene sequence similarities and 77.6–92.8 % recA gene sequence similarities, respectively. ANI, is DDH and AAI values between strain X0750 T and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8 % respectively, confirming that strain X0750 T represents a novel species within the genus Weissella . Based upon the data obtained in the present study, a novel species, Weissella sagaensis sp. nov., is proposed and the type strain is X0750 T (=NCIMB 15192 T =CCM 8924 T =LMG 31184 T =CCTCC AB 2018403 T ).