Weissella sagaensis sp. nov., isolated from traditional Chinese yogurt

Three Gram-stain-positive bacterial strains, designated X0750 T , X0278 and X0401, isolated from traditional yogurt in Tibet Autonomous Region, PR China, were characterized by a polyphasic approach, including sequence analyses of the 16S rRNA gene and three housekeeping genes ( pheS , rpoA and recA ), determination of average nucleotide identity (ANI) and average amino acid identity (AAI), in silico DNA–DNA hybridization ( is DDH), fatty acid methyl ester (FAME) analysis and phenotypic characterization. Strain X0750 T was phylogenetically related to the type strains of Weissella hellenica , Weissella bombi , Weissella paramesenteroides , Weissella jogaejeotgali , Weissella thailandensis , Weissella oryzae , Weissella cibaria and Weissella confusa , having 94.4–100 % 16S rRNA gene sequence similarities, 76.7–90.0 %  pheS gene sequence similarities, 88.9–99.4 %  rpoA gene sequence similarities and 77.6–92.8 %  recA gene sequence similarities, respectively. ANI, is DDH and AAI values between strain X0750 T and type strains of phylogenetically related species were less than 90.4, 40.9 and 92.8 % respectively, confirming that strain X0750 T represents a novel species within the genus Weissella . Based upon the data obtained in the present study, a novel species, Weissella sagaensis sp. nov., is proposed and the type strain is X0750 T (=NCIMB 15192 T =CCM 8924 T =LMG 31184 T =CCTCC AB 2018403 T ).