Open AccessReclassification of Clostridium diolis Biebl and Spröer 2003 as a later heterotypic synonym of Clostridium beijerinckii Donker 1926 (Approved Lists 1980) emend. Keis et al. 2001
Author(s) -
Hiromichi Kobayashi,
Yasuhiro Tanizawa,
Mitsuo Sakamoto,
Yasukazu Nakamura,
Moriya Ohkuma,
Masanori Tohno
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004059
Subject(s) - biology , clostridium , synonym (taxonomy) , clostridium beijerinckii , microbiology and biotechnology , clostridium infections , zoology , bacteria , clostridium difficile , genetics , genus , antibiotics
Clostridium diolis shares high similarity based on 16S rRNA gene sequences and fatty acid composition with Clostridium beijerinckii . In this study, the taxonomic status of C. diolis was clarified using genomic and phenotypic approaches. High similarity was detected among C. diolis DSM 15410 T , C. beijerinckii DSM 791 T and NCTC 13035 T , showing average nucleotide identity on blast and in silico DNA–DNA hybridization values over 97 and 85 %, respectively. Results of investigations for substrate utilization and enzyme activity displayed no striking differences between C. diolis DSM 15410 T and C. beijerinckii JCM 1390 T . Based on the results, we propose the reclassification of Clostridium diolis as a later heterotypic synonym of Clostridium beijerinckii . The type strain is ATCC 25752 T (=CIP 104308 T =DSM 791 T =JCM 1390 T =LMG 5716 T =NCTC 13035 T ).