Open AccessExiguobacterium flavidum sp. nov., isolated from the Red Maple Lake
Author(s) -
Xue Meng,
Ying Chang,
Lichun Zhou,
ZongJun Du
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004048
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , strain (injury) , phylogenetic tree , botany , microbiology and biotechnology , bacteria , cell wall , gene , biochemistry , genetics , anatomy
A Gram-stain-positive, motile, facultatively anaerobic, non-sporing, and rod-shaped bacterial strain, designated HF60 T , was isolated from the Red Maple Lake of Guizhou Province, China. The DNA G+C content of the strain HF60 T was 55.0 %. The predominant isoprenoid quinones were identified as MK-7 (56.4 %) and MK-8 (35.7 %). The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and aminophosphoglycolipid. The major fatty acids were anteiso-C 13 : 0 , iso-C 15 : 0 , C 16 : 0 and iso-C 13 : 0 . The strain had cell wall peptidoglycan type A3α l -Lys-Gly. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain HF60 T belonged to the genus Exiguobacterium and was most closely related to Exiguobacterium sibiricum JCM 13490 T (97.2 % 16S rRNA gene sequence similarity), followed by Exiguobacterium undae DSM 14481 T (97.1 %), Exiguobacterium antarcticum DSM 14480 T (96.9 %) and Exiguobacterium aurantiacum NBRC 14763 T (94.5 %). The differential phenotypic properties, together with the phylogenetic and genetic distinctiveness indicated that strain HF60 T can be considered to represent a novel species of the genus Exiguobacterium , for which the name Exiguobacterium flavidum sp. nov. is proposed, The type strain is HF60 T (=MCCC 1H00336 T =KCTC 33987 T ).