Georgenia wutianyii sp. nov. and Georgenia yuyongxinii sp. nov. isolated from plateau pika (Ochotona curzoniae) on the Qinghai–Tibet plateau of China

Four novel bacterial strains, designated Z294 T , Z311, Z443 T and Z446, were isolated from the intestinal contents of plateau pika ( Ochotona curzoniae ) on the Qinghai–Tibet Plateau of China. Cells were Gram-stain-positive, catalase-positive, oxidase-negative, aerobic, non-motile and short-rod shaped. Phylogenetic analyses based on 16S rRNA gene sequences indicated that the four isolates belong to the genus Georgenia , but clearly separate from the currently recognized species. Both type strains (Z294 T and Z443 T ) shared low 16S rRNA gene sequence similarity, digital DNA–DNA hybridization relatedness and average nucleotide identity values with Georginia satyanarayanai NBRC 107612 T , G. subflava JCM 19765 T , G. ruanii JCM 15130 T and G. thermotolerans DSM 21501 T and against each other. The genomic DNA G+C contents of strains Z294 T and Z443 T were 73.3 and 70 %, respectively. The major cellular fatty acids of strain Z294 T were anteiso -C 15 : 0 , anteiso -C 15 : 1 A and C 16 : 0 , in contrast to anteiso -C 15 : 0 and anteiso -C 15 : 1 A for strain Z443 T . Both type strains (Z294 T and Z443 T ) shared the following common features: glucose, rhamnose and ribose as cell-wall sugars; MK-8(H 4 ) as major menaquinone; alanine, glutamic acid and lysine as cell-wall amino acids; and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phosphoglycolipid as polar lipids. Comparing the phenotypic and phylogenetic features among the four strains and their related organisms, strains Z294 T and Z443 T represent two novel species within the genus Georgenia , for which the names Georgenia wutianyii sp. nov. (type strain Z294 T =CGMCC 1.16428 T =DSM 106344 T ) and Georgenia yuyongxinii sp. nov. (type strain Z443 T =CGMCC 1.16435 T =DSM 106174 T ) are proposed.