Tabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis

A novel Gram-negative, obligately aerobic, rod-shaped and non-motile bacterium, designated strain K13M18 T , was isolated from the intestinal tract of a Korean indigenous fish, oily bitterling ( Acheilognathus koreensis ). Strain K13M18 T formed creamy-pink colonies on a marine agar plate. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indicated that strain K13M18 T was most closely related to Tabrizicola sediminis DRYC-M-16 T , sharing 97.62 % similarity with that strain. Strain K13M18 T belonged to the genus Tabrizicola , which formed a cluster with Tabrizicola aquatica RCRI19 T , Tabrizicola fusiformis SY72 T , Tabrizicola sediminis DRYC-M-16 T and Tabrizicola alkalilacus DJC T in a phylogenetic tree based on the 16S rRNA gene sequences. Strain K13M18 T grown optimally in 0 % (w/v) NaCl, at pH 7 and 30 °C, in a marine broth medium. The predominant cellular fatty acids were C 18 : 1 ω 7 c and C 18 : 1 ω 6 c . The major respiratory isoprenoid quinone was ubiquinone Q-10. Polar lipids of strain K13M18 T contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, six unidentified aminophospholipids, one unidentified aminolipid and an unidentified lipid. Based on genome sequencing, the DNA G+C content of strain K13M18 T was 64.08 mol %, with an average nucleotide identity value, calculated by a comparative genomic analysis of strains K13M18 T and T. sediminis DRYC-M-16 T , of 74.82 %. Based on the phylogenetic, genotypic, and phenotypic information, strain K13M18 T is proposed to be a novel species of the genus Tabrizicola . The type strain is K13M18 T (=KCTC 62659 T =JCM 33230 T ).