Open AccessTabrizicola piscis sp. nov., isolated from the intestinal tract of a Korean indigenous freshwater fish, Acheilognathus koreensis
Author(s) -
Jeong Eun Han,
Woorim Kang,
June-Young Lee,
Hojun Sung,
Dong-Wook Hyun,
Hyun Sik Kim,
Pil Soo Kim,
Euon Jung Tak,
Yun-Seok Jeong,
Jae Yun Lee,
SoYeon Lee,
Ji-Hyun Yun,
Myeong Ho Jung,
Na-Ri Shin,
Tae Woong Whon,
Myung-Suk Kang,
KiEun Lee,
Byoung-Hee Lee,
Jin-Woo Bae
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004034
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , microbiology and biotechnology , genomic dna , ribosomal rna , genus , bacteria , gene , botany , genetics , anatomy
A novel Gram-negative, obligately aerobic, rod-shaped and non-motile bacterium, designated strain K13M18 T , was isolated from the intestinal tract of a Korean indigenous fish, oily bitterling ( Acheilognathus koreensis ). Strain K13M18 T formed creamy-pink colonies on a marine agar plate. Results of phylogenetic analysis based on the 16S rRNA gene sequence similarity indicated that strain K13M18 T was most closely related to Tabrizicola sediminis DRYC-M-16 T , sharing 97.62 % similarity with that strain. Strain K13M18 T belonged to the genus Tabrizicola , which formed a cluster with Tabrizicola aquatica RCRI19 T , Tabrizicola fusiformis SY72 T , Tabrizicola sediminis DRYC-M-16 T and Tabrizicola alkalilacus DJC T in a phylogenetic tree based on the 16S rRNA gene sequences. Strain K13M18 T grown optimally in 0 % (w/v) NaCl, at pH 7 and 30 °C, in a marine broth medium. The predominant cellular fatty acids were C 18 : 1 ω 7 c and C 18 : 1 ω 6 c . The major respiratory isoprenoid quinone was ubiquinone Q-10. Polar lipids of strain K13M18 T contained phosphatidylcholine, phosphatidylglycerol, phosphatidylinositol, diphosphatidylglycerol, six unidentified aminophospholipids, one unidentified aminolipid and an unidentified lipid. Based on genome sequencing, the DNA G+C content of strain K13M18 T was 64.08 mol %, with an average nucleotide identity value, calculated by a comparative genomic analysis of strains K13M18 T and T. sediminis DRYC-M-16 T , of 74.82 %. Based on the phylogenetic, genotypic, and phenotypic information, strain K13M18 T is proposed to be a novel species of the genus Tabrizicola . The type strain is K13M18 T (=KCTC 62659 T =JCM 33230 T ).