Open AccessElioraea rosea sp. nov., a plant promoting bacterium isolated from floodwater of a paddy field
Author(s) -
Hyo Jin Lee,
Kyung-Sook Whang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004028
Subject(s) - biology , paddy field , bacteria , botany , agronomy , genetics
A Gram-stain-negative bacterium, designated strain PF-30 T , was isolated from floodwater of a paddy field in South Korea. Strain PF-30 T was found to be a strictly aerobic, motile and pink-pigmented rods which can grow at 25–40 °C (optimum, 28 °C), at pH 5.0–9.0 (optimum pH 7.0) and at salinities of 0.5–3.0 % NaCl (optimum 0.5 % NaCl). Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain PF-30 T belongs to the genus Elioraea , showing highest sequence similarity to Elioraea tepidiphila TU-7 T (97.1%) and less than 91.3 % similarity with other members of the family Acetobacteraceae . The average nucleotide identity (ANI) and DNA–DNA relatedness between the strain PF-30 T and E. tepidiphila TU-7 T yielded an ANI value of 75.1 % and DNA–DNA relatedness of 11.7±0.7 %, respectively. The major fatty acids were identified as C 18 : 0 and C 18 : 1 ω 7 c . The predominant respiratory quinone was identified as Q-10. The DNA G+C content was determined to be 69.9 mol%. The strain PF-30 T was observed to produce plant-growth-promoting materials such as indole-3-acetic acid (IAA), siderophore and phytase. On the basis of the results from phylogenetic, chemotaxonomic and phenotypic data, we concluded that strain PF-30 T represents a novel species of the genus Elioraea , for which the name Elioraea rosea sp. nov. is proposed. The type strain is PF-30 T (=KACC 19985 T =NBRC 113984 T ).