Open AccessPhragmitibacter flavus gen. nov., sp. nov. a new member of the family Verrucomicrobiaceae
Author(s) -
Sára Szuróczki,
Gorkhmaz Abbaszade,
Attila Szabó,
Károly Bóka,
Peter Schümann,
Erika Tóth
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004025
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , microbiology and biotechnology , verrucomicrobia , phosphatidylglycerol , oxidase test , glycolipid , phylogenetics , bacteria , botany , gene , bacteroidetes , genetics , biochemistry , phospholipid , enzyme , anatomy , membrane , phosphatidylcholine
The Gram-stain-negative, aerobic, non-motile, oxidase- and catalase-positive, rod-shaped yellow-coloured bacterial strain MG-N-17 T was isolated from a water sample of Lake Fertő/Neusiedler See (Hungary). Results of phylogenetic analysis based on the 16S rRNA gene sequence revealed that the strain forms a distinct linage within the family Verrucomicrobiaceae of the phylum Verrucomicrobia , and its closest relatives are Verrucomicrobium spinosum DSM 4136 T (94.38 %) and Roseimicrobium gellanilyticum DC2a-G7 T (91.55 %). The novel bacterial strain prefers a weak alkaline environment and grows optimally between 22–28 °C in the absence of NaCl. The major isoprenoid quinones are MK-10, MK-11, MK-12 and MK-9. The major cellular fatty acids are anteiso-C 15 : 0 , C 16 : 0 , C 16 : 1 ω5 c and iso-C 14 : 0 . The polar lipid profile contains phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids and four unidentified glycolipids. The assembled draft genome of strain MG-N-17 T had 44 contigs with an N50 value 348255 nt, 56.5× genome coverage, total length of 5 910 933 bp and G+C content of 56.9 mol%. Strain MG-N-17 T (=DSM 106674 T =NCAIM B.02643 T ) is proposed as the type strain of a new genus and species in the family Verrucomicrobiaceae , for which the name Phragmitibacter flavus gen. nov., sp. nov. is proposed.