Open AccessNiastella caeni sp. nov., isolated from activated sludge
Author(s) -
Mengyao Sheng,
Yang Zhou,
Xiao Yang,
Jianyi Xu,
Jiguo Qiu,
Jun He,
Shanlian Qiu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.004017
Subject(s) - 16s ribosomal rna , biology , strain (injury) , phylogenetic tree , bacteria , microbiology and biotechnology , gene , biochemistry , genetics , anatomy
A Gram-stain-negative, aerobic, non-flagellated and filamentous-shaped bacterium, HX-16-21 T , was isolated from activated sludge. Strain HX-16-21 T was able to degrade gentisate, protocatechuic acid and p -hydroxybenzoic acid and herbicides quizalofop-p-ethyl and diclofop-methyl. The strain shared 97.2 % 16S rRNA gene sequence similarity to Niastella vici CCTCC AB 2015052 T and less than 97 % similarities to other type strains. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain HX-16-21 T belonged to the genus Niastella and formed a subclade with N. vici CCTCC AB 2015052 T . The major polar lipids were phosphatidylethanolamine, phosphatidylcholine and six unidentified lipids. The major fatty acids were iso-C 15:0 , iso-C 15:1 G and iso-C 17:0 3-OH. The predominant respiratory quinone was menaquinone 7 (MK-7). The draft genome of strain HX-16-21 T was 8.1 Mb, and the G+C content was 43.5 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain HX-16-21 T and N. vici CCTCC AB 2015052 T were 80.6 and 26.8 %, respectively. Based on both phenotypic and phylogenetic evidence, strain HX-16-21 T is considered to represent a novel species in the genus Niastella , for which the name Niastella caeni sp. nov. is proposed. The type strain is HX-16-21 T (=KCTC 72288 T =ACCC 61580 T ).