Mangrovimonas spongiae sp. nov., a novel member of the genus Mangrovimonas isolated from marine sponge

A taxonomic study was carried out on strain HN-E26 T , which was isolated from sponge collected from Yangpu Bay, Hainan, PR China. Cells of strain HN-E26 T were Gram-stain-negative, motile by gliding, yellow-pigmented and rod-shaped. The strain could grow at 10–40 °C (optimum, 25 °C), at pH 6.0–9.0 (optimum, pH 7.0) and in 0.5–12 % (w/v) NaCl (optimum, 4–7 %). This isolate was positive for oxidase, catalase, and the hydrolysis of starch, xylan, aesculin and gelatin, but negative for indole production and the reduction of nitrate. Strain HN-E26 T shared the highest 16S rRNA gene sequence similarity with Mangrovimonas yunxiaonensis LYYY01 T (95.5 %), followed by Formosa spongicola A2 T (94.4 %), Meridianimaribacter flavus NH57N T (94.3 %) and Winogradskyella exilis 022-2-26 T (94.3 %). The phylogenetic tree based on 16S rRNA gene sequences revealed that strain HN-E26 T formed a distinct phylogenetic lineage within the cluster comprising Mangrovimonas yunxiaonensis LYYY01 T and ‘ Mangrovimonas xylaniphaga ’ ST2L12 T . The dominant fatty acids were iso-C 15 : 0 and iso-C 15 : 1 G. The major polar lipids comprised phosphatidylethanolamine, three unidentified aminolipids and six unidentified lipids. The respiratory lipoquinone was identified as MK-6. The G+C content of the genomic DNA was 33.9 mol%. Based on the phenotypic and phylogenetic data, strain HN-E26 T represents a novel species of the genus Mangrovimonas , for which the name Mangrovimonas spongiae sp. nov. is proposed, with the type strain HN-E26 T (=MCCC 1K03326 T =LMG 30458 T ).