Sphingobacterium olei sp. nov., isolated from oil-contaminated soil

A Gram-stain-negative, rod-shaped, non-motile and non-spore-forming bacterium, designated HAL-9 T , was isolated from oil-contaminated soil in Daqing oilfield, Heilongjiang Province, PR China. Strain HAL-9 T was able to degrade quizalofop- p -ethyl and diclofop-methyl. Growth was observed at 10–35 °C (optimum, 30 °C), pH 6.0–10.0 (optimum, pH 7.0) and salinity of 0 %–5.0 % (w/v; optimum 1.0 %). The results of phylogenetic analysis based on the 16S rRNA gene indicated that strain HAL-9 T belongs to the genus Sphingobacterium and showed the highest sequence similarity (98.3 %) to Sphingobacterium alkalisoli Y3L14 T , followed by Sphingobacterium mizutaii DSM 11724 T (95.1 %) and Sphingobacterium lactis DSM 22361 T (95.1 %). Menaquinone-7 (MK-7) was the only isoprenoid quinone. The predominant cellular fatty acids were summed feature 3 (C 16 : 1 ω 7 c and/or C 16 : 1 ω 6 c ), iso-C 15: 0 and iso-C 17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, three phosphoglycolipids and three unidentified lipids. The draft genome of strain HAL-9 T was 5.41 Mb. The G+C content of strain HAL-9 T was 40.6 mol%. Furthermore, the average nucleotide identity and in silico DNA–DNA hybridization values between strain HAL-9 T and S. alkalisoli Y3L14 T were 86.2 % and 32.8 %, respectively, which were below the standard thresholds for species differentiation. On the basis of phenotypic, genotypic and phylogenetic evidence, strain HAL-9 T represents a novel species in the genus Sphingobacterium , for which the name Sphingobacterium olei sp. nov. is proposed. The type strain is HAL-9 T (=ACCC 61581 T =CCTCC AB 2019176 T =KCTC 72287 T ).