Open AccessPsychrobacillus glaciei sp. nov., a psychrotolerant species isolated from an Antarctic iceberg
Author(s) -
Jun Young Choi,
Pyung Cheon Lee
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003998
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , phylogenetics , genomic dna , ribosomal rna , whole genome sequencing , bacteria , microbiology and biotechnology , gene , genetics , genome , anatomy
We performed taxonomic studies on a psychrotolerant strain, designated PB01 T , isolated from an Antarctic iceberg. The cells of strain PB01 T were Gram-stain-positive, strictly aerobic, white–yellow and rod-shaped. The results of 16S rRNA gene sequence analysis revealed that strain PB01 T was closely related to Psychrobacillus psychrodurans DSM 11713 T (99.19 % similarity), Psychrobacillus psychrotolerans DSM 11706 T (98.91 %) and Psychrobacillus insolitus DSM 5 T (98.85 %). Despite high 16S rRNA gene sequence similarity, the degrees of DNA–DNA relatedness between strain PB01 T and its three closest phylogenetic neighbours were 62.4±7.3 % for P. psychrodurans DSM 11713 T , 61.1±5.4 % for P. psychrotolerans DSM 11706 T and 56.1±6.9 % for P. insolitus DSM 5 T . The predominant cellular fatty acids were anteiso-C 15 : 0 , iso-C 15 : 0 and C16 : 1ω7с-OH. Menaquinone-8 was the major respiratory quinone, and phosphatidylethanolamine was the major polar lipid. The DNA G+C content of strain PB01 T calculated from the complete genome sequence was 36.0 mol%. Based on the phenotypic, chemotaxonomic, genomic and phylogenetic data obtained in the present study, we conclude that strain PB01 T represents a novel species of the genus Psychrobacillus , for which we propose the name Psychrobacillus glaciei sp. nov. The type strain is PB01 T (=CECT 9792 T =KCTC 43041 T ).