Paenibacillus paridis sp. nov., an endophytic bacterial species isolated from the root of Paris polyphylla Smith var. yunnanensis

A Gram-reaction-positive, endospore-forming and rod-shaped bacterial strain, designated py1325 T , was isolated from the root of Paris polyphylla Smith var. yunnanensis collected from Yunnan Province, PR China, and subjected to a polyphasic taxonomic characterization. It grew optimally with 0–1 % NaCl (w/v), at pH 7 and at 30 °C. The major respiratory quinone was MK-7 and the diagnostic diamino acid in the cell-wall peptidoglycan was meso -diaminopimelic acid. The major cellular fatty acid was anteiso-C 15 : 0 . The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, two unidentified aminophospholipids, two unidentified phospholipids and two unidentified lipids. The results of 16S rRNA gene sequence analysis revealed the highest levels of sequence similarity with respect to Paenibacillus luteus R-3 T (99.0 %), Paenibacillus sinopodophylli CCTCC AB 2016047 T (97.9 %), Paenibacillus castaneae DSM 19417 T (97.5 %) and Paenibacillus endophyticus LMG 27297 T (97.2 %). The digital DNA–DNA hybridization and averagenucleotide identity values between py1325 T and these species ranged 20.6–53.3 % and 79.9–93.6 %. The G+C content of the genomic DNA was 47.7 mol%. According to the phylogenetic, phenotypic and chemotaxonomic evidence, strain py1325 T clearly represents a novel species of the genus Paenibacillus , for which the name Paenibacillus paridis sp. nov. is proposed. The type strain is py1325 T (=CCTCC AB 2015220 T =LMG 29068 T ).