Cryobacterium ruanii sp. nov. and Cryobacterium breve sp. nov., isolated from glaciers

Strains Sr36 T and TMT4-23 T were isolated from No. 1 glacier in Xinjiang Uygur Autonomous Region and Toumingmengke glacier in Gansu Province, PR China, respectively. They were Gram-stain-positive and rod-shaped micro-organisms. The optimum growth temperature of the two strains was 10–14 °C. Phylogenetic analysis showed that the two strains were related to members of the genus Cryobacterium . The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between strain Sr36 T and its close relatives Cryobacterium luteum Hh15 T , Cryobacterium aureum Hh31 T , Cryobacterium levicorallinum Hh34 T and Cryobacterium flavum Hh8 T were 81.16–87.24 and 28.0–32.5 %, respectively. The ANI and dDDH values between strain TMT4-23 T and its close relative Cryobacterium psychrotolerans 0549 T were 81.16 and 22.3 %. The polar lipids of strain Sr36 T were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid and three unidentified lipids. The polar lipids of strain TMT4-23 T were diphosphatidylglycerol, phosphatidylglycerol, one unidentified glycolipid, one unidentified phospholipid and six unidentified lipids. The major fatty acids of strain Sr36 T were anteiso-C 15 : 0 , iso-C 16 : 0 , anteiso-C 17 : 0 and anteiso-C 15 : 1 . The major fatty acids of strain TMT4-23 T were anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 , anteiso-C 15 : 1 and iso-C 15 : 1 . Both strains contained 2,4-diaminobutyric acid and their predominant menaquinone was MK-10. On the basis of the phenotypic, phylogenetic and genotypic data, two novel species Cryobacterium ruanii sp. nov. (type strain = Sr36 T =CGMCC 1.9275 T =NBRC 113797 T ) and Cryobacterium breve sp. nov. (type strain =TMT4-23 T =CGMCC 1.9556 T =NBRC 113800 T ) are proposed.