Corynebacterium suranareeae sp. nov., a glutamate producing bacterium isolated from soil and its complete genome-based analysis

Strain N24 T was isolated from soil contaminated with starling’s feces collected from Roi-Et province, Thailand. Cells of N24 T were Gram-stain-positive rods, aerobic and non-spore-forming. N24 T was positive for catalase, urease, citrate utilization, nitrate reduction and Methyl Red (MR) test but negative for oxidase, casein, gelatin liquefaction, tyrosine, Voges–Proskauer (VP) reaction and starch hydrolysis. Meso -diaminopimelic acid, rhamnose, ribose, arabinose and galactose were detected in its whole-cell hydrolysates. The results of the 16S rRNA gene sequence analysis indicated that N24 T represented a member of the genus Corynebacterium . N24 T was closely related to Corynebacterium glutamicum ATCC 13032 T , with 99.0 % 16S rRNA gene sequence similarity. According to results obtained using in silico DNA–DNA hybridization approaches, N24 T showed highest DNA–DNA relatedness (27.6 %) and average nucleotide identity (84.1 %) to Corynebacterium glutamicum ATCC 13032 T . The DNA G+C content of N24 T was 51.8 mol% (genome based). The major cellular fatty acids of N24 T were C 16 : 0 , and C 18 : 1 ω9 c . N24 T had the nine isoprenes unit, MK-9(H 2 ) as the predominant menaquinone. The predominant polar lipids were phosphatidylglycerol, phosphatidylinositol and diphosphatidylglycerol. Mycolic acids were also present. According to the complete genome sequence data, strain N24 T and C. glutamicum ATCC 13032 T are close phylogenetic neighbours, but have different genome characteristics. On the basis of the results of the genotypic and genomic studies and phenotypic characteristics including chemotaxonomy, strain N24 T should be classified as representing a novel species of the genus Corynebacterium , for which the name Corynebacterium suranareeae sp. nov. is proposed. The type strain is N24 T (TBRC 5845 T =NBRC 113465 T ).