Open AccessSaccharothrix deserti sp. nov., an actinomycete isolated from desert soil
Author(s) -
Jinjuan Liu,
Yong Sun,
Jinrong Liu,
Yue Wu,
Changqun Cao,
Rongpeng Li,
Jihong Jiang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003989
Subject(s) - biology , botany , desert (philosophy) , soil microbiology , microbiology and biotechnology , ecology , soil water , philosophy , epistemology
A Gram-stain-positive, aerobic actinomycete, designated strain BMP B8144 T , was isolated from desert soil, in Xinjiang province, northwest China. The isolate produced scanty aerial mycelium and fragmented substrate mycelium on most tested media. Cell-wall hydrolysates contained meso -diaminopimelic acid, galactose and mannose. The diagnostic phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylhydroxylethanolamine, phosphatidylinositol, and phosphatidylinositol mannosides. The major fatty acids included iso -C 16 : 0 , C 17 : 1 ω 8 c and iso -C 15 : 0 . The predominant menaquinones were MK-9(H 4 ) and MK-10(H 4 ). The DNA G+C content was 70.4 mol% (genome). Based on the 16S rRNA gene sequence analysis on EzBioCloud server, strain BMP B8144 T showed the closest similarities to Saccharothrix lopnurensis YIM LPA2h T (98.9 %) and ‘ Saccharothrix yanglingensis ’ Hhs.015 (98.6 %). However, it can be distinguished from the closest strains based on the low levels of DNA–DNA relatedness (59.3±1.8 and 47.9±2.3 %, respectively). A combination of morphological, chemotaxonomic and phylogenetic characteristics, strain BMP B8144 T represents a novel species of the genus Saccharothrix , for which the name Saccharothrix deserti sp. nov. is proposed. The type strain is BMP B8144 T (=CGMCC 4.7490 T =KCTC 49001 T ).