Open AccessNatrialba swarupiae sp. nov., a halophilic archaeon isolated from a hypersaline lake in India
Author(s) -
Swapnil Kajale,
Neelima Deshpande,
Snigdha Pali,
Yogesh S. Shouche,
Avinash Sharma
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003986
Subject(s) - biology , 16s ribosomal rna , halophile , strain (injury) , lysis , phosphatidylglycerol , microbiology and biotechnology , bacteria , ribosomal rna , distilled water , genome size , gene , biochemistry , genome , chromatography , chemistry , phospholipid , genetics , anatomy , membrane , phosphatidylcholine
A haloarchaeal strain (ESP3B_9 T ) was isolated from the salt pan of Sambhar salt lake, Rajasthan, India. Cells were coccoid, non-motile, Gram-stain-negative and formed reddish-pink pigmented colonies. The strain was aerobic, able to grow at 35–55 °C (optimum, 40 °C), in 20–35 % (25 %) NaCl and pH 8–10 (pH 9). Mg 2+ not required for growth. The cells were lysed in distilled water and the minimum NaCl concentration that prevented cell lysis was 5 % w/v. The 16S rRNA gene sequence similarities between strain ESP3B_9 T and Natrialba hulunbeirensis JCM 10989 T and Natrialba magadii ATCC 43099 T were 96.53 and 96.25 % respectively. The similarities of the RNA polymerase subunit B gene between strain ESP3B_9 T and N. hulunbeirensis JCM 10989 T and N. magadii ATCC 43099 T were 84.47 and 84.9 % respectively. Genome sequencing revealed a genome size of 4.20 Mbp with DNA G+C content of 62.5 mol%. The major polar lipids were phosphotidylglycerol and phosphatidylglycerol phosphate methyl esters with minor amounts of unidentified lipids. The results of polyphasic analysis determined that strain ESP3B_9 T represents a novel species of the genus Natrialba , for which the name Natrialba swarupiae sp. nov. is proposed. The type strain is ESP3B_9 T (MCC 3419 T =JCM 33002 T =KCTC 4279 T =CGMCC 1.16737 T ).