Open AccessPseudolysobacter antarcticus gen. nov., sp. nov., isolated from soil in Fildes Peninsula, Antarctica
Author(s) -
Ziyan Wei,
Tong Wu,
Yao Huang,
Guoxin Zhu,
Yumin Zhang,
Yingchao Geng,
Fang Peng
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003984
Subject(s) - 16s ribosomal rna , biology , phylogenetic tree , strain (injury) , microbiology and biotechnology , bacteria , gene , biochemistry , genetics , anatomy
A novel strain, designated AQ6-296 T , was isolated from a soil sample collected in Fildes Peninsula, Antarctic. Cells were Gram-stain-negative, non-endospore-forming, non-motile, strictly aerobic and rod-shaped. Growth occurred at 4–28 °C (optimum, 20 °C) and at pH 6.0–7.0 (optimum, pH 7.0). NaCl was not obligatory for growth. Colonies were pale yellow after growth for 3 days at 20 °C on Reasoner's 2A agar. The strain was weakly positive for oxidase and the catalase test was negative. The only respiratory quinone was Q-8. The predominant cellular fatty acids were iso-C 16 : 0 , iso-C 15 : 0 , iso-C 11 : 0 3OH, summed feature 3 (comprising C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ) and summed feature 9 (comprising iso-C 17 : 1 ω9 c and/or C 16 : 0 10-methyl). The major polar lipids were phosphatidylethanolamine, unknown aminolipids, phosphatidylglycerol and diphosphatidylglycerol. The results of phylogenetic analysis based on 16S rRNA gene sequences (the highest similarity at 92.4 % to Lysobacter dokdonensis ) indicated that strain AQ6-296 T is within the family Xanthomonadaceae . The DNA G+C content of the type strain was 58.6 mol%. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain AQ6-296 T is considered to represent a novel genus and species in the family Xanthomonadaceae , for which the name Pseudolysobacter antarcticus gen. nov., sp. nov. is proposed. The type strain is AQ6-296 T (CCTCC AB 2016313 T =KCTC 52744 T ).