Open AccessMumia zhuanghuii sp. nov., isolated from the intestinal contents of plateau pika (Ochotona curzoniae) on the Qinghai–Tibet Plateau
Author(s) -
Zhi Tian,
Shan Lu,
Dong Jin,
Jing Yang,
Ji Pu,
XinHe Lai,
Junqin Li,
Sihui Zhang,
Kui Dong,
Wenjing Lei,
Ying Huang,
Zhihong Ren,
Dezhu Zhang,
Suping Wang,
Jianguo Xu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003979
Subject(s) - biology , pika , 16s ribosomal rna , phylogenetic tree , diamino acid , microbiology and biotechnology , strain (injury) , bacteria , gene , genetics , ecology , gene sequence , national park , anatomy
Two Gram-staining-positive, catalase-positive, oxidase-negative, aerobic, non-motile, irregular rod-shaped bacterial strains (Z350 T and Z527) were isolated from intestinal contents of plateau pika ( Ochotona curzoniae ) from the Qinghai–Tibet Plateau, PR China. Results of phylogenetic analyses based on 16S rRNA gene sequences indicated that strain Z350 T belongs to the genus Mumia (family Nocardioidaceae ) but clearly differs from the currently recognized species Mumia xiangluensis DSM 101040 T (98.4 % similarity) and Mumia flava DSM 27763 T (97.4 %). Strain Z350 T had a DNA G+C content of 70.7 mol% and shared 80.4 and 76.7 % average nucleotide identity values and 23.4 and 20.6 % in silico DNA–DNA hybridization relatedness with M. xiangluensis DSM 101040 T and M. flava DSM 27763 T , respectively. Further phylogenetic analyses based on 497 core genes indicated that our isolates were members of the genus Mumia but separated from all existing genera within the family Nocardioidaceae . The major cellular fatty acids were C 18 : 1 ω9 c and 10-methyl C 18 : 0 . The cell wall contained ll -diaminopimelic acid as the diamino acid, and rhamnose, ribose and glucose as whole cell-wall sugars. MK-9(H 4 ) was detected as the major menaquinone. Polar lipids present were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unidentified phospholipid. Based on distinct differences in the genotypic and phenotypic data from the two Mumia species, a novel species, Mumia zhuanghuii sp. nov., is proposed. The type strain is Z350 T (=CGMCC 4.7464 T =DSM 106288 T ).