Open AccessAidingimonas lacisalsi sp. nov., isolated from a salt lake in China
Author(s) -
Tongwei Guan,
Li-Sha Tian,
ShuKun Tang,
Lei Tian,
Yu-Zhou Feng
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003955
Subject(s) - biology , 16s ribosomal rna , strain (injury) , halophile , botany , phylogenetic tree , genus , bacteria , gene , genetics , anatomy
A novel bacterium, XHU 5135 T , belonging to the genus Aidingimonas , was isolated from a salt lake sample collected in Xinjiang Province, north-west PR China. The isolate was Gram-stain-negative, rod-shaped and non-motile. The strain was catalase-positive and oxidase-negative. Growth occurred at NaCl concentrations of 5–25 % (optimum, 10–13 %), at 13–41 °C (35–37 °C) and at pH 6.0–10.0 (pH 7.0–8.0). The predominant ubiquinone was Q-9. The major fatty acids were C 19 : 0 cyclo ω 8 c and C 16 : 0 . The G+C content of the genomic DNA was 58.1 mol%. The affiliation of strain XHU 5135 T with the genus Aidingimonas was confirmed by 16S rRNA gene sequence comparisons. The closest type strain was Aidingimonas halophile YIM 90637 T , which showed a 16S rRNA gene sequence similarity of 97.5 %. The ANI value between XHU 5135 T and the closest type strain was 80.01 %. The estimated digital DNA–DNA hybridization estimate value between strain XHU 5135 T and the closest type strain was 22.80 %. Phenotypically, the characteristics of XHU 5135 T were shown to differ from the most closely related species, A. halophila . On the basis of the data from this polyphasic study, strain XHU 5135 T represents a novel species of the genus Aidingimonas , for which the name Aidingimonas lacisalsi sp. nov. is proposed. The type strain is strain XHU 5135 T (=CCTCC AB 2016344 T =KCTC 42945 T =DSM 104700 T ).