Open AccessMuricauda alvinocaridis sp. nov., isolated from shrimp gill from the Okinawa Trough
Author(s) -
Lijun Liu,
Min Yu,
Shun Zhou,
Tianyu Fu,
Wentao Sun,
Long Wang,
Xiaohua Zhang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003953
Subject(s) - 16s ribosomal rna , biology , shrimp , strain (injury) , bacteria , bacteroidetes , genomic dna , microbiology and biotechnology , dna , biochemistry , anatomy , genetics , ecology
A Gram-stain-negative, strictly aerobic, non-motile, long, straight-rod and non-flagellated marine bacterium strain, designated SCR12 T , was isolated from the gill of a shrimp collected in the Tangyin hydrothermal field of the Okinawa Trough. The growth temperature was in the range of 16–40 °C and the optimum temperature was 37 °C. Optimal growth occurred at pH 6.5 and in the presence of 3 % (w/v) NaCl. The predominant isoprenoid quinone of strain SCR12 T was menaquinone-6 (MK-6). The predominant fatty acids (>10 %) were iso-C 15 : 0 (44.2 %), iso-C 15 : 1 G (19.0 %) and iso-C 17 : 0 3-OH (12.1 %). The major polar lipids comprised one phosphatidylethanolamine, three unidentified phospholipids, two unidentified aminolipids and three unidentified lipids. Based on the results of 16S rRNA gene sequence analysis, strain SCR12 T was found to be most closely related to Muricauda olearia CL-SS4 T (98.09 %), followed by Muricauda beolgyonensis BB-My12 T (97.65 %), Muricauda aquimarina SW-63 T (97.58 %) and Muricauda ruestringensis DSM 13258 T (97.31 %) and with lower sequence similarities (95.74–97.10 %) to other species of the genus Muricauda . Genome relatedness between strain SCR12 T and M. olearia CL-SS4 T was computed using both average nucleotide identity (ANI) and DNA–DNA hybridization (DDH) and resulted in values of 85.6 % and 29.3±2.3 %, respectively. The genomic DNA G+C content of strain SCR12 T was 42.3 mol%. On the basis of polyphasic analysis, the strain SCR12 T was considered to represent a novel species of the genus Muricauda, for which the name Muricauda alvinocaridis sp. nov. is proposed. The type strain is SCR12 T (=MCCC 1K03731 T =JCM 33425 T ).