Open AccessMicrobulbifer harenosus sp. nov., an alginate-degrading bacterium isolated from coastal sand
Author(s) -
Huiqin Huang,
Kunlian Mo,
Yonghua Hu,
Min Liu,
Jun Zhu,
Xuefeng Zou,
Siqi Bao
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003945
Subject(s) - biology , 16s ribosomal rna , microbiology and biotechnology , genomic dna , strain (injury) , bacteria , sequence analysis , ribosomal rna , gene , genetics , anatomy
A Gram-stain-negative, aerobic, rod-shaped bacterium with peritrichous flagella, designated strain HB161719 T , was isolated from coastal sand collected from Tanmen Port in Hainan, PR China. The isolate was found to grow with 2–11 % (w/v) NaCl, at 15–45 °C and pH 6.0–10.0, with an optima of 2–3 % NaCl, 37 °C and pH 7.0, respectively. Chemotaxonomic analysis showed that Q-8 was detected as the sole respiratory quinone and that iso-C 15 : 0 and summed features 3, 8 and 9 were the major cellular fatty acids. The G+C content of the genomic DNA was 58.2 mol%. Analysis of the 16S rRNA gene sequence of the strain showed an affiliation with the genus Microbulbifer , sharing 98.7, 98.4, 97.8 and 97.8 % sequence similarities to the closest relatives of Microbulbifer okinawensis ABABA23 T , Microbulbifer pacificus SPO729 T , Microbulbifer taiwanensis CC-LN1-12 T and Microbulbifer gwangyangensis GY2 T , respectively. Low DNA–DNA hybridization values showed that it formed a distinct genomic species. The combined phenotypic and molecular features supported that strain HB161719 T represents a novel species of the genus Microbulbifer , for which the name Microbulbifer harenosus sp. nov. is proposed. The type strain is HB161719 T (=CGMCC 1.13584 T =JCM 32688 T ).