Open AccessSkermanella pratensis sp. nov., isolated from meadow soil, and emended description of the genus Skermanella
Author(s) -
Qiannan Guo,
Zhengfu Zhou,
Lijuan Zhang,
Chen Zhang,
Ming Chen,
Bo Wang,
Min Lin,
Wei Wang,
Wei Zhang,
Xufeng Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003944
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , phosphatidylglycerol , genomic dna , botany , genus , ribosomal rna , microbiology and biotechnology , gene , phosphatidylcholine , genetics , anatomy , phospholipid , membrane
A novel Gram-stain-negative, light pink-coloured, short rod-shaped, designated strain W17 T , was isolated from a meadow soil sample collected from Xinjiang, PR China. The 16S rRNA gene sequence analysis indicated that strain W17 T was related most closely to Skermanella rosea M1 T (98.72 %) and Skermanella mucosa 8-14-6 T (98.44 %). However, strain W17 T showed a low level of DNA–DNA relatedness to S. rosea M1 T (32.4±2.6 %) and S. mucosa 8-14-6 T (33.5±0.1 %). The genome size of the novel strain was 5.87 Mb and the genomic DNA G+C content was 67.27 mol%. The only respiratory quinone of strain W17 T was Q-10. Diphosphatidylglycerol, phosphatidylglycerol. phosphatidylethanolamine and phosphatidylcholine were the major polar lipids. The predominant cellular fatty acids were C 18 : 1 ω6 c and/or C 18 : 1 ω7 c (48.53 %), C 16 : 0 (20.88 %) and C 18 : 0 (14.92 %). The phylogenetic, phenotypic and chemotaxonomic data showed that strain W17 T represents a novel species of the genus Skermanella , for which the name Skermanella pratensis sp. nov. is proposed. The type strain is W17 T (=GDMCC 1.1392 T =KCTC 62434 T ).