Pseudomonas carnis sp. nov., isolated from meat

During investigations of spoilage-associated meat microbiota, Pseudomonas isolates were found in two different laboratories showing highest similarities to Pseudomonas lactis DSM 29167 T , Pseudomonas paralactis DSM 29164 T and Pseudomonas azotoformans DSM 18862 T based on 16S rRNA gene sequence comparisons. Phylogenetic analysis of the complete rpoB gene sequences of isolates B4-1 T and SpeckC indicated a separate branch with 99.0 and 99.1 % identity, respectively, to their closest relative ( P. lactis DSM 29167 T ). Further phenotypic and chemotaxonomic characterizations, as well as average nucleotide identity (ANIb) values obtained from the draft genomes, revealed that these isolates could be considered as representing a novel species, with ANIb values of around 94 and 90 % with their closest relatives P. lactis and P. paralactis . Other related species showed ANIb values below 90 %, including Pseudomonas libanensis DSM 17149 T , Pseudomonas synxantha DSM 18928 T , Pseudomonas orientalis DSM 17489 T , Pseudomonas veronii DSM 11331 T and P. azotoformans DSM 18862 T . Genome-to-genome distance calculations between B4-1 T and its closest relative, P. lactis DSM 29167 T , showed 62.6 % relatedness. The G+C contents of B4-1 T and SpeckC were 59.8 and 59.9 mol%, respectively. The major cellular lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol; the major quinone was Q9. Based on these data, the new species Pseudomonas carnis sp. nov. is proposed, the type strain is B4-1 T (=DSM 107652 T =LMG 30892 T ); a second strain is SpeckC (=DSM 107651=LMG 30893).