Open AccessGlycomyces buryatensis sp. nov., an actinobacterium isolated from steppe soil
Author(s) -
E. P. Nikitina,
Shaowei Liu,
Fei-Na Li,
L. B. Buyantueva,
E. Yu. Abidueva,
ChengHang Sun
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003923
Subject(s) - biology , steppe , botany , ecology
A novel actinobacterial strain, designated 18 T , was isolated from a steppe soil sample collected in Buryatia, Russia and subjected to polyphasic taxonomic characterization. The strain was aerobic and Gram-stain-positive. The 16S rRNA gene sequence of strain 18 T exhibited highest similarity to Glycomyces paridis CPCC 204357 T (97.2 %). Results of phylogenetic analysis showed that strain 18 T formed a distinct branch clearly affiliated to the genus Glycomyces . Whole-cell hydrolysates of the isolate contained meso -diaminopimelic acid as the cell-wall diamino acid. The whole-cell sugar profile was found to contain galactose, glucose, ribose and xylose. MK-10(H 4 ) and MK-11(H 4 ) were the predominant menaquinones. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, five unidentified phospholipids and four unidentified polar lipids. The major fatty acids identified were anteiso-C 15 : 0 and iso-C 15 : 0 . The G+C content of the genomic DNA of strain 18 T was 68.0 mol% (draft genome sequence). Based on its phylogenetic, phenotypic and chemotaxonomic features, strain 18 T was considered to represent a novel species of the genus Glycomyces , for which the name Glycomyces buryatensis sp. nov. is proposed. The type strain of Glycomyces buryatensis is 18 T (=JCM 33362 T =CGMCC 4.7610 T ).