Open AccessRheinheimera sediminis sp. nov., a marine bacterium isolated from coastal sediment
Author(s) -
Qunfang Yu,
Yanxiang Qi,
Zaixing He,
Pu Jing
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003917
Subject(s) - biology , 16s ribosomal rna , bacteria , strain (injury) , phylogenetic tree , microbiology and biotechnology , gene , genetics , anatomy
A Gram-stain-negative, aerobic, rod-shaped bacterium, designated strain YQF-1 T , was isolated from coastal sediment in Jiangsu Province (PR China) and characterized phylogenetically and phenotypically. Bacterial optimal growth occurred at 28 °C (range 4–40 °C) and pH 7 (range pH 6–11). Phylogenetic analysis based on 16S rRNA gene sequence indicated that YQF-1 T was related to members of the genus Rheinheimera and shared the highest sequence identities with Rheinheimera mesophila DSM 29723 T (98.5 %), followed by Rheinheimera tangshanensis DSM 19460 T (98.4 %), Rheinheimera tilapiae Ruye-90 T (97.9 %), Rheinheimera soli BD-d46 T (97.9 %), Rheinheimera aquatica GR5 T (97.4 %), Rheinheimera coerulea TAPG2 T (97.3 %) and Rheinheimera texasensis A62-14B T (97.1 %). The 16S rRNA gene sequence identities between YQF-1 T and other members of the genus Rheinheimera were below 97.0 %. The digital DNA–DNA hybridization value between YQF-1 T and Rheinheimera mesophila DSM 29723 T was 25.1±2.3 %. The average nucleotide identity (ANI) value between YQF-1 T and Rheinheimera mesophila DSM 29723 T was 81.4 %. The major respiratory quinone was Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, phospholipid, two unidentified aminolipids and three unidentified lipids. The strain had summed feature 8 (C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 16 : 0 , summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), iso-C 15 : 0 , and anteiso-C 17 : 1 ω9 c as the major fatty acids. The G+C content of the genomic DNA was 46.2 mol%. On the basis of phenotypic, genotypic and phylogenetic evidence, strain YQF-1 T represents a novel species of the genus Rheinheimera , for which the name Rheinheimera sediminis sp. nov. is proposed, with the type strain YQF-1 T (=KCTC 72183 T =MCCC 1K03646 T ).