Open AccessElioraea thermophila sp. nov., a thermophilic bacterium from hot spring of the class Alphaproteobacteria, emended description of the genus Elioraea and proposal of Elioraeaceae fam. nov.
Author(s) -
Neeli Habib,
Inam Ullah Khan,
Min Xiao,
Mohammad Saeid Hejazi,
XiaoYang Zhi,
WenJun Li
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003915
Subject(s) - biology , 16s ribosomal rna , thermophile , phylogenetic tree , alphaproteobacteria , microbiology and biotechnology , ribosomal rna , strain (injury) , genus , bacteria , genomic dna , botany , gene , genetics , anatomy
A Gram-staining-negative, aerobic, curved rod-shaped and thermophilic bacterial strain, designated YIM 72297 T , was isolated from a sediment sample collected from a hot spring in Tengchong county, Yunnan province, south-west China. Growth was observed at pH 5.0–9.0 with an optimum of pH 7.0–7.5, and at 45–60 °C with an optimum of 55 °C. Positive for catalase and oxidase. The 16S rRNA gene sequence comparison indicated that strain YIM 72297 T was most closely related to Elioraea tepidiphila DSM 17972 T (96.9 %) and showed <91 % sequence similarities to members of the order Rhodospirillales . Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain YIM 72297 T formed a distinct lineage within the genus Elioraea , and revealed that the genus Elioraea formed a novel family-level clade in the order Rhodospirillales . The ANI and the dDNA-DNA hybridization estimate values between strains YIM 72297 T and Elioraea tepidiphila DSM 17972 T were 70.8 and 20.1 %, respectively. Strain YIM 72297 T contained Q-10 as the predominant ubiquinone. The major fatty acids (>5 %) were summed C 18 : 0 (35.8 %), summed feature 8 (30.1 %), C 16 : 0 (12.6 %), C 18 : 1 2OH (5.6 %) and C 16 : 0 2OH (5.4 %). The polar lipids consisted of phosphatidylcholine, phosphatidylethanolamine, diphosphosphatidylglycerol and phosphatidylglycerol in addition to two unidentified aminolipids. The DNA G+C content of YIM 72297 T was 70.8 mol% (draft genome). On the basis of the polyphasic taxonomic evidence presented in this study, strain YIM 72297 T should be classified as representing a novel species of the genus Elioraea , for which the name Elioraea thermophila sp. nov. is proposed, with the type strain YIM 72297 T (=CCTCC AB 2017169 T =KCTC 62323 T ). In addition, a novel family, Elioraeaceae fam. nov., is proposed to accommodate the genus Elioraea .