Open AccessThalassocella blandensis gen. nov., sp. nov., a novel member of the family Cellvibrionaceae
Author(s) -
Teresa Lucena,
David R. Arahal,
Isabel Sanz-Sáez,
Silvia G. Acinas,
Olga Sánchez,
Rosa Aznar,
Carlos PedrósAlió,
María J. Pujalte
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003906
Subject(s) - biology , halophile , 16s ribosomal rna , gammaproteobacteria , strain (injury) , oxidase test , bacteria , biochemistry , alphaproteobacteria , microbiology and biotechnology , gene , enzyme , genetics , anatomy
Strain ISS155 T , isolated from surface Mediterranean seawater, has cells that are Gram-reaction-negative, motile, strictly aerobic chemoorganotrophic, oxidase-positive, unable to reduce nitrate to nitrite, and able to grow with cellulose as the sole carbon and energy source. It is mesophilic, neutrophilic, slightly halophilic and has a requirement for sodium and magnesium ions. Its 16S rRNA gene sequence places the strain among members of Cellvibrionaceae , in the Gammaproteobacteria , with Agarilytica rhodophyticola 017 T as closest relative (94.3 % similarity). Its major cellular fatty acids are C 18 : 1 , C 16 : 0 and C 16 : 1 ; major phospholipids are phosphatidyl glycerol, phosphatidyl ethanolamine and an unidentified lipid, and the major respiratory quinone is Q8. The genome size is 6.09 Mbp and G+C content is 45.2 mol%. A phylogenomic analysis using UBCG merges strain ISS155 T in a clade with A. rhodophyticola , Teredinibacter turnerae , Saccharophagus degradans and Agaribacterium haliotis type strain genomes, all of them possessing a varied array of carbohydrate-active enzymes and the potential for polysaccharide degradation. Average amino acid identity indexes determined against available Cellvibrionaceae type strain genomes show that strain ISS155 T is related to them by values lower than 60 %, with a maximum of 58 % to A. rhodophyticola 017 T and 57 % to T. turnerae T7902 T and S. degradans 2-40 T . These results, together with the low 16S rRNA gene sequence similarities and differences in phenotypic profiles, indicate that strain ISS155 T represents a new genus and species in Cellvibrionaceae , for which we propose the name Thalassocella blandensis gen. nov., sp. nov., and strain ISS155 T (=CECT 9533 T =LMG 31237 T ) as the type strain.