Pseudooceanicola onchidii sp. nov., isolated from a marine invertebrate from the South China Sea

A novel bacterial strain, XY-99 T , was isolated from the epidermis of a marine invertebrate of the genus Onchidium from seawater of the South China Sea. The cells of the strain were aerobic, Gram-stain-negative, non-motile, and oval-shaped (0.8–1.0 µm wide and 1.0–1.5 µm long) without a flagellum. The strain grew at temperatures of 15–37 °C (optimum, 35–37 °C), at pH 5.5–9.5 (optimum 7.5), and at NaCl concentrations of 0–12.0 % (w/v) (optimum 1.5–3.0 %). The major fatty acids (>10 %) were summed feature 8 (comprising C 18 : 1 ω7 c and/or C 18 : 1 ω6 c ), C 16 : 0 and 11-methyl C 18 : 1 ω7 c . The predominant polar lipid was diphosphatidylglycerol. The respiratory quinone was Q-10. The closet phylogenetic neighbours were Pseudooceanicola flagellatus DY470 T and Pseudooceanicola nitratireducens JLT1210 T , showing 97.5 and 97.3 % of 16 s rRNA gene sequence similarity. The genome size of XY-99 T was 3 673 499 bp, with 64.5 % DNA G+C content. The average nucleotide identity and digital DNA–DNA hybridization values between XY-99 T and Pseudooceanicola flagellatus DY470 T were 72.8 and 14.0 %, respectively, while they were 79.3 and 22.3 % between XY-99 T and Pseudooceanicola nitratireducens JLT1210 T . Characterization based on phylogenetic, phenotypic, chemotaxonomic and genomic evidence demonstrated that XY-99 T represents a novel species of the genus Pseudooceanicola , for which the name Pseudooceanicola onchidii sp. nov. is proposed. The type strain is XY-99 T (=KCTC 72211 T =MCCC 1K03607 T ).