Open AccessLactobacillus mulieris sp. nov., a new species of Lactobacillus delbrueckii group
Author(s) -
Júlio César Rocha,
João Botelho,
Magdalena Ksiezarek,
Svetlana Ugarčina Perović,
Miguel P. Machado,
João André Carriço,
Lígia L. Pimentel,
Ana Sofia Salsinha,
Luís M. RodriguezAlcalá,
Manuela Pintado,
Teresa Gonçalves Ribeiro,
Luı́sa Peixe
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003901
Subject(s) - biology , 16s ribosomal rna , lactobacillus , phylogenetic tree , microbiology and biotechnology , strain (injury) , whole genome sequencing , phylogenetics , sequence analysis , genome , genetics , gene , bacteria , anatomy
One Gram-stain-positive, non-motile, non-spore-forming, catalase-negative, and coccobacilli-shaped strain, designated c10Ua161M T , was isolated from a urine sample from a reproductive-age healthy woman. Comparative 16S rRNA gene sequence analysis indicated that strain c10Ua161M T belonged to the genus Lactobacillus . Phylogenetic analysis based on pheS and rpoA gene sequences strongly supported a clade encompassing strains c10Ua161M T and eight other strains from public databases, distinct from currently recognized species of the genus Lactobacillus. In silico Average Nucleotide Identity (ANI) and Genome-to-Genome Distance Calculator (GGDC), showed 87.9 and 34.3 % identity to the closest relative Lactobacillus jensenii , respectively. The major fatty acids of strain c10Ua161M T were C 18 : 1 ω 9c (65.0%), C 16 : 0 (17.8%), and summed feature 8 (10.2 %; comprising C 18 : 1 ω7 c , and/or C 18 : 1 ω6 c ). The DNA G+C content of the strains is 34.2 mol%. On the basis of data presented here, strain c10Ua161M T represents a novel species of the genus Lactobacillus , for which the name Lactobacillus mulieris sp. nov. is proposed. The type strain is c10Ua161M T (=CECT 9755 T =DSM 108704 T ).