Open AccessMarinobacter fonticola sp. nov., isolated from deep sea cold seep sediment
Author(s) -
Qinglei Sun,
Yong Sun,
Yuanyuan Sun,
Zhaokun Luan,
Chao Lian
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003895
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , microbiology and biotechnology , botany , bacteria , gene , biochemistry , anatomy , genetics
In this study, we report a novel Gram-negative bacterium, designated as strain CS412 T , isolated from deep-sea sediment collected in a cold seep area of the South China Sea. Growth of strain CS412 T occurred at 4–40 °C (optimum, 28 °C), pH 5.0–11.0 (optimum, pH 6.0) and with 0–19 % (w/v) NaCl (optimum, 1–2 %). Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain CS412 T belonged to the genus Marinobacter . The closest phylogenetic neighbours of strain CS412 T were Marinobacter pelagius HS225 T (96.9 %), Marinobacter szutsaonensis NTU-104 T (96.8%), Marinobacter santoriniensis NKSG1 T (96.4%) and Marinobacter koreensis dd -M3 T (96.3 %). The genomic DNA G+C content of strain CS412 T was 58.0 mol%. The principal respiratory quinone was ubiquinone-9 (Q-9). The polar lipids of CS412 T contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, aminophospholipidand and four glycolipids. The major fatty acids of CS412 T contained cyclo-C 19 : 0 ω8 c , C 16 : 0 , C 18 : 1 ω7 c and C 18 : 1 ω7 c 11-methyl. The results of phylogenetic, physiological, biochemical and morphological analyses suggested that strain CS412 T represents a novel species of the genus Marinobacter , and the name Marinobacter fonticola sp. nov. is proposed with the type species CS412 T (=CCTCC AB 2019197 T =KCTC 72475 T ).