Open AccessCumulibacter soli sp. nov., isolated from farmland soil
Author(s) -
Qinqin Gao,
Wankui Jiang,
Gao-Jie Sun,
Lu Zhang,
Mingliang Zhang,
Xiaoan Li,
Zhijian Ke,
Yidong Zhou,
Qing Hong,
Jianhong Xu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003893
Subject(s) - biology , 16s ribosomal rna , strain (injury) , phylogenetic tree , genomic dna , bacteria , diamino acid , genome size , glycolipid , microbiology and biotechnology , gene , phylogenetics , diaminopimelic acid , fatty acid , genome , biochemistry , genetics , gene sequence , anatomy
A Gram-stain-positive, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterium, designated as strain G-1 T , was isolated from farmland soil sampled in in Fuyang, Anhui Province, PR China. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G-1 T was closely related to Cumulibacter manganitolerans 2-36 T (97.7 % similarity). Strain G-1 T contained iso-C 16 : 0 , C 17 : 1 ω6 c , iso-C 15 : 0 and iso-C 14 : 0 as the predominant fatty acids. The polar lipids of strain G-1 T were diphosphatidylglycerol, phosphatidylethanolamine, an unidentified phospholipid, an unidentified lipid and two unidentified glycolipids. The predominant respiratory quinone of strain G-1 T was MK-9(H 4 ). The cell wall contained meso -diaminopimelic acid as the diagnostic diamino acid. The G+C content of the genomic DNA based on genome calculations was 64.2 mol%. Average nucleotide identity and the digital DNA–DNA hybridization values for the draft genomes between strain G-1 T and strain 2-36 T were 75.7 and 20.2 %, respectively. On the basis of phenotypic and phylogenetic data, strain G-1 T is considered to represent a novel species of the genus Cumulibacter , for which the name Cumulibacter soli sp. nov. is proposed. The type strain is G-1 T (=CCTCC AB2019021 T =KCTC 49258 T ).