Open AccessPseudorivibacter rhizosphaerae gen. nov., sp. nov., isolated from rhizosphere soil of Camellia sinensis (L.) O. Ktze and emended description of the genus Rivibacter
Author(s) -
Xing-Kui Zhou,
Qingqing Li,
Limin Dong,
Deng-Qiang Yang,
Lan Liu,
BaoZhu Fang,
Che Ok Jeon,
Wenjun Li,
Yan-Qing Duan
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003878
Subject(s) - biology , 16s ribosomal rna , rhizosphere , botany , phylogenetic tree , strain (injury) , oxidase test , ribosomal rna , bacteria , microbiology and biotechnology , gene , genetics , biochemistry , anatomy , enzyme
A Gram-stain-negative, facultative anaerobic, motile and straight rod-shaped bacterium, designated strain C1-9 T , was isolated from rhizosphere soil of Camellia sinensis (L.) O. Ktze collected from a tea garden in Huize, south-western PR China. Cells were oxidase-positive and catalase-negative. Growth occurred at 20–40 °C and pH 6.0–10.0, with an optimal growth at 30 °C and pH 7.0. The respiratory quinone was detected as ubiquinone-8 (Q-8). The major fatty acids were identified as summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), C 16 : 0 and summed feature 8 (C 18 : 1 ω7 c or C 18 : 1 ω6 c ). The cellular polar lipids contained phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, three unidentified phospholipids, two unidentified lipids, one unidentified aminophospholipid and one unidentified aminolipid. The polyamine types were detected as 1,8-diaminooctane and 2-hydroxyputrescine. The genomic DNA G+C content was 68.6 mol%. Based on the results of 16S rRNA gene sequence analysis, strain C1-9 T (MF687442) showed highest sequence similarity to Rivibacter subsaxonicus DSM 19570 T (97.1 %). The phylogenetic tree based on 16S rRNA gene sequences showed that strain C1-9 T clustered close to R. subsaxonicus DSM 19570 T , Methylibium petroleiphilum CCTCC AB 2014193 T and species belonging to the genera Rhizobacter and Piscinibacter . The phylogenomic tree indicated that strain C1-9 T formed a clade with R. subsaxonicus . The average nucleotide identity value was 76.0 % between strain C1-9 T and R. subsaxonicus DSM 19570 T , which is lower than the prokaryotic species delineation threshold of 95.0–96.0 %. The polyphasic taxonomic characteristics indicated that strain C1-9 T represents a novel species of a new genus within the order Burkholderiales , for which the name Pseudorivibacter rhizosphaerae gen. nov., sp. nov. (type strain C1-9 T = KCTC 62325 T =CGMCC 1.13864 T ) is proposed.