Open AccessDyadobacter flavalbus sp. nov., isolated from lake sediment
Author(s) -
Jianhang Qu,
Yi-Fei Yue,
Jia Zhou,
Lingbo Qu,
Linfeng Wang
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003876
Subject(s) - biology , 16s ribosomal rna , phylogenetic tree , strain (injury) , genomic dna , gene , contig , genome , whole genome sequencing , ribosomal rna , genetics , anatomy
A novel bacterial strain, designated NS28 T , was isolated from interfacial sediment sampled at Taihu Lake, PR China. Cells were rod-shaped, Gram-negative, aerobic and non-motile on Reasoner's 2A medium. The results of phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NS28 T was most closely related to species from the genus Dyadobacter , with 98.4 and 96.0 % 16S rRNA gene sequence similarity to its closest phylogenetic neighbours Dyadobacter sediminis CGMCC 1.12895 T and Dyadobacter luticola CCTCC AB 2017091 T , respectively. MK-7 was the only cellular menaquinone. The major fatty acids were summed feature 3 (C 16 : 1 ω7 c and/or C 16 : 1 ω6 c ), iso-C 15 : 0 and C 16 : 1 ω5 c . The major polar lipids were phosphatidylethanolamine, one phospholipid, one aminolipid, one lipid and two unidentified lipids. Genomic analysis of strain NS28 T indicated that the total genome size was 6 477 094 bp with a G+C content of 44.8 mol%, 5380 protein-coding genes, 79 contigs and an N50 length of 299584 bp. On the basis of the genomic DNA sequence, the average nucleotide identity values were 90.5 and 74.1 % with D. sediminis CGMCC 1.12895 T and D. luticola CCTCC AB 2017091 T , respectively. Digital DNA-DNA hybridization results of strain NS28 T with D. sediminis CGMCC 1.12895 T and D. luticola CCTCC AB 2017091 T were 40.9 and 18.6 %, respectively. Based on the phenotypic, chemotaxonomic, phylogenetic and genome sequence data presented here, it is proposed that strain NS28 T represents a novel species of the genus Dyadobacter for which the name Dyadobacter flavalbus is proposed . The type strain is NS28 T (=NBRC 113854 T =MCCC 1K03764 T ).