Open AccessFerrovibrio terrae sp. nov., isolated from soil
Author(s) -
Dajung Ryu,
Minji Kim,
Bit Han,
KiEun Lee,
Byoung-Hee Lee,
EunYoung Lee,
Gi-Yong Jung,
So-Jeong Kim,
Soo-Je Park
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003872
Subject(s) - biology
Designated strain K5 T was isolated from soil on Jeju Island. The bacterium was aerobic, Gram-stain-negative, oxidase-positive, catalase-low activity, motile, short-rod shaped, opaque and formed white colonies that were circular, raised and had entire margins. Strain K5 T was able to grow at 15–40 °C, pH 4–9 and at 0–2 % (w/v) NaCl concentration. Phylogenetic analysis based on its 16S rRNA gene sequences indicated that strain K5 T is closely related to Ferrovibrio soli A15 T (98.9 % sequence similarity), Ferrovibrio denitrificans Sp-1 T (98.7 %) and Ferrovibrio xuzhouensis LM-6T (97.4 %). The sole respiratory quinone was determined to be ubiquinone-10. The dominant fatty acids of strain K5 T were summed feature 8 (C 18 : 1 ω 7c / C 18 : 1 ω 6c , 29.8 %), C 19 : 0 cyclo ω8 c (20.2 %) and C 16 : 0 (24.4 %). DNA G+C content was 63.6 % and DNA–DNA relatedness between strain K5 T and other three members of the genus Ferrovibrio ranged from 24 to 28 %. The major polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine and aminolipids. Moreover, polyphasic characterization revealed that strain K5 T represents a novel species in the genus Ferrovibrio, for which the name Ferrovibrio terrae sp. nov. is proposed. The type strain is K5 T (=KCCM 43295 T =LMG 30611 T ).