Open AccessFlavobacterium psychrotolerans sp. nov., a psychrotolerant bacterium isolated from Renlongba glacier on the Tibetan Plateau
Author(s) -
Leilei Yang,
Qing Liu,
HongCan Liu,
YuGuang Zhou,
YuHua Xin
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003868
Subject(s) - biology , 16s ribosomal rna , flavobacterium , phylogenetic tree , microbiology and biotechnology , bacteria , strain (injury) , flavobacteriaceae , phylogenetics , genus , gene , botany , genetics , bacteroidetes , pseudomonas , anatomy
One aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain RB1R5 T , was isolated from Renlongba glacier in Tibet Autonomous Region, China. Growth was observed at 4–25 °C and pH 7.0–8.0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain RB1R5 T belongs to the genus Flavobacterium , and its closest relatives are Flavobacterium luteum IMCC26026 T and Flavobacterium psychroterrae CCM 8827 T with 97.75 and 97.42% gene sequence similarities, respectively. The average nucleotide identity and DNA–DNA hybridization values between strain RB1R5 T and its closest relative Flavobacterium luteum IMCC26026 T were 78.02 and 21.8 %, respectively. The major cellular fatty acids were anteiso-C 15 : 0 and summed feature 3 (comprising C 16:1 ω7 c /C 16:1 ω6 c ). The sole menaquinone was MK-6. The major phospholipid was phosphatidylethanolamine. On the basis of phenotypic, phylogenetic and chemotaxonomic characterizations, strain RB1R5 T represents a novel species of the genus Flavobacterium , for which the name Flavobacterium psychrotolerans sp. nov. is proposed. The type strain is RB1R5 T (=CGMCC 1.23024 T =NBRC 113060 T ).