Open AccessLuteimonas yindakuii sp. nov. isolated from the leaves of Dandelion (Taraxacum officinale) on the Qinghai-Tibetan Plateau
Author(s) -
Sihui Zhang,
Xiaoxia Wang,
Jing Yang,
Shan Lu,
XinHe Lai,
Dong Jin,
Ji Pu,
Junqin Li,
YuYuan Huang,
Zhang Gui,
Wentao Zhu,
Ying Huang,
Zhi Tian,
Jia-Rong Zhou,
Kui Dong,
Wenjing Lei,
Zhihong Ren,
Suping Wang,
Jianguo Xu
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003863
Subject(s) - biology , 16s ribosomal rna , botany , taraxacum officinale , phylogenetic tree , bacteria , microbiology and biotechnology , dandelion , biochemistry , gene , medicine , alternative medicine , traditional chinese medicine , pathology , genetics
Two strains (S-1072 T and 1626) of Gram-stain-negative, oxidase- and catalase-positive, aerobic, rod-shaped, motile bacteria with a single polar flagellum, were isolated from the leaves of Dandelion ( Taraxacum officinale ) on the Qinghai–Tibet Plateau of China. The cells grew optimally at 28 °C, pH 7.0 and with 0.5 % (w/v) NaCl on brain–heart infusion agar. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains S-1072 T and 1626 belong to the genus Luteimonas , sharing the highest similarity with Luteimonas arsenica CCTCC AB 2014326 T (97.0 %), Luteimonas terricola CGMCC 1.8985 T (96.9 %) and Luteimonas aestuarii KCTC 22048 T (96.6 %). The phylogenomic tree indicated that strains S-1072 T and 1626 were most closely related to Luteimonas abyssi CGMCC 1.12611 T . The biochemical characteristics revealed that strains S-1072 T and 1626 could neither produce trypsin nor produce acid from d -glucose, N -acetylglucosamine and maltose, distinguishing them from four closest relatives. The DNA G+C contents of strains S-1072 T and 1626 were 69.2 and 69.3 mol% respectively. The digital DNA–DNA hybridization values of our isolates with their four closely related species were below the 70 % threshold. The predominant menaquinone was Q-8 (98.7 %) and the major polar lipids included diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major fatty acids (>10 %) were iso-C 15 : 0 , iso-C 16 : 0 and summed feature 9 (10-methyl C 16 : 0 and/or iso-C 17 : 1 ω 9 c ). Based on the data obtained, strains S-1072 T and 1626 should be classified as a novel species of the genus Luteimonas , for which the name Luteimonas yindakuii sp. nov. is proposed. The type strain is S-1072 T (=CGMCC 1.13927 T =JCM 33487 T ).