Open AccessPaenibacillus protaetiae sp. nov., isolated from gut of larva of Protaetia brevitarsis seulensis
Author(s) -
Jun Heo,
Soojin Kim,
Jeong-Seon Kim,
SeungBeom Hong,
Soon-Wo Kwon
Publication year - 2020
Publication title -
international journal of systematic and evolutionary microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.925
H-Index - 173
eISSN - 1466-5034
pISSN - 1466-5026
DOI - 10.1099/ijsem.0.003860
Subject(s) - biology , 16s ribosomal rna , peptidoglycan , diamino acid , microbiology and biotechnology , paenibacillus , strain (injury) , phylogenetic tree , bacteria , ribosomal rna , botany , cell wall , gene sequence , biochemistry , gene , genetics , anatomy
A Gram-stain-positive, rod-shaped, strictly aerobic, endospore-forming and motile bacterium with peritrichous flagella was isolated from a gut sample of the larva of Protaetia brevitarsis seulensis at the National Institute of Agricultural Sciences, Wanju-gun, Republic of Korea. Growth was observed at 15–50 °C (optimum, 28–37 °C), pH 6.0–8.0 (pH 7.0) and only without NaCl. 16S rRNA gene sequence comparisons indicated that strain FW100M-2 T had the highest similarity to type strains of Paenibacillus thailandensis S3-4A T (96.8 %) and Paenibacillus agaridevorans DSM 1355 T (96.3 %), and had sequence similarity values less than 96.0 % to all other taxa. The phylogenetic tree showed that strain FW100M-2 T fell into the genus Paenibacillus , and formed a cluster with P. thailandensis S3-4A T independent from other Paenibacillus species. Antesio-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 were detected as the major fatty acids. The only isoprenoid quinone was MK-7. Polar lipids of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, an unidentified aminophospholipid, two unidentified phospholipids and an unidentified lipid were present. The meso -diaminopimelic acid was present in the cell-wall peptidoglycan. The genomic DNA G+C content was 51.5 mol%. Hence, strain FW100M-2 T represents a novel species of the genus Paenibacillus , for which the name Paenibacillus protaetiae sp. nov. is proposed, with FW100M-2 T (=KACC 19327 T =NBRC 113071 T ) as the type strain.